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Determination of double bond position in wax esters by dimethyl disulfide derivatization and mass spectrometry
Háková, Martina ; Schwarzová, Karolina (advisor) ; Coufal, Pavel (referee)
Wax esters are substantial constituents of natural waxes, which can be found in many living organisms. Properties of lipids, including wax esters, may be significantly influenced by the position of double bond. In this diploma thesis the location of double bonds was determined by dimethyl disulfide (DMDS) derivatization followed by detection using tandem mass spectrometry with atmospheric pressure chemical ionization (APCI) and electrospray ionization (ESI). We managed to measure the APCI and ESI MS/MS spectra of 8 different wax esters with different position of double bond. Diagnostic ions determining double bond position were identified. This method could be used in HPLC/MS analysis of wax esters, which cannot be analyzed by GC/MS. It was shown that the DMDS derivatization reaction and mass detection with APCI ionization is also suitable for locating double bonds in alkenes.
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Utilization of mass spectrometry and other analytical techniques for analysis of rhenium complexes
Vaňátková, Petra ; Jelínek, Ivan (advisor) ; Smrček, Stanislav (referee)
Rhenium complexes coordinated with suitable organic ligand gain increased interest in radiopharmaceutical medicine. Besides suitable radiological properties given by a metal ion itself, detailed knowledge of overall chemical properties of formed complexes, namely their exact structures, chemical stabilities and possible degradation pathways are essential pre-requisites for their clinical application. Selected rhenium complexes with pyrogallol 2,3-dihydroxynaphthalene and catechol as strongly bound ligands were prepared by a reaction of tetrabutylammonium tetrachlorooxorhenate with twofold molar excess of ligand in presence of various amount of triethylamine. The structures of formed complexes and their consequent reaction products were estimated by means of mass spectrometry with electrospray ionization. The kinetics of reactions in course of complex formation and consequent decomposition were primarily followed by UV- Vis absorption spectra measurement, complemented by single or continuous electrospray mass spectrometry analyses. Optimized HPLC and CZE procedures were utilized for monitoring of the composition of reaction mixtures in course of Re complex formation and for purity check of prepared complexes. Semi-preparative HPLC mode was succesfully utilized for isolation and final purification of...
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Determination of double bond position in wax esters by dimethyl disulfide derivatization and mass spectrometry
Háková, Martina ; Schwarzová, Karolina (advisor) ; Coufal, Pavel (referee)
Wax esters are substantial constituents of natural waxes, which can be found in many living organisms. Properties of lipids, including wax esters, may be significantly influenced by the position of double bond. In this diploma thesis the location of double bonds was determined by dimethyl disulfide (DMDS) derivatization followed by detection using tandem mass spectrometry with atmospheric pressure chemical ionization (APCI) and electrospray ionization (ESI). We managed to measure the APCI and ESI MS/MS spectra of 8 different wax esters with different position of double bond. Diagnostic ions determining double bond position were identified. This method could be used in HPLC/MS analysis of wax esters, which cannot be analyzed by GC/MS. It was shown that the DMDS derivatization reaction and mass detection with APCI ionization is also suitable for locating double bonds in alkenes.
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Monitoring of water use, druought and yield impacts of winter wheat using imaginery from satellites
Jurečka, František ; Anderson, M. ; Hlavinka, Petr ; Semerádová, Daniela ; Trnka, Miroslav ; Hain, C. ; Gao, F. ; Yang, Y. ; Žalud, Zdeněk
Remote sensing can be very useful tool for drought monitoring, providing valuable information about yield-limiting moisture conditions and crop response under current climate conditions. In this study the Atmosphere-land Exchange Inverse (ALEXI) model was used. The ALEXI model uses the morning surface temperature (LST) rise and provides information on the surface moisture status. In this paper correlations between yields and satellite indicators of crop water use or evapotranspiration (ET) were studied for the period 2002-2014. Correlations were studied for winter wheat at district scale in Vysocina, Jihomoravsky and Olomoucky regions since winter wheat is one of the traditional and most important crops grown in these regions. The Evaporative Stress Index (ESI) was used for these correlations as an ET-based index. Time series of Pearson correlation coefficient (r) computed between ESI and winter wheat yields at district scale were analysed. Strongest correlations are associated with districts within the Southern Moravian lowlands in Jihomoravsky and Olomoucky region, where frequency of occurrence of severe drought was highest over the period of record. Severe drought resulted in significant yield impacts, particularly in years 2003 and 2012. Correlations tend to be lower over the highlands districts of Vysocina and surroundings. In these districts, yields are more temperature than moisture limited and were more stable over the period of record.
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